How to interpret the Variant Allele Frequency??
I am new to the field of Bioinformatics and seeking guidance. I am currently in the process of calculating the Variant Allele Frequency (VAF) using the VarScan software. I have obtained the results and have included screenshots for reference. I would greatly appreciate assistance in understanding how to interpret these results.
These are the Headings of the result
CHROM POS ID REF ALT QUAL FILTER INFO FORMAT Sample1
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2 answers
Variant allele frequency (VAF) = Depth at Altered Allele / Total Depth
Total depth = Depth at Altered Allele + Depth at Reference Allele
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