Differential gene expression analysis after transcriptome assembly with SPAdes
Hello.
I'm was recommended to use SPAdes for transcriptome assembly. I didn't find explanation on quantification and differential expression analysis after the assembly in the program's documentation, Does anybody have any recommendations for proper programs?
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Istvan Albert
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langziv •
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langziv •
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As GenoMax points out follow any protocol that operates on the same type of information (in this case transcriptome data).
At the same time do account for the potential of using a more noisy and less accurate representation. Basically spend more time on validating the various counts and alignments/classifications.
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