VarDictJava - teststrandbias.R
Hi all,
I am trying to optimise my use of VarDictJava for some FFPE sequenced samples with a custom targeted panel. So, I release the following code for implementation:
bashArguments = "/apps/VARDICTJAVA/1.8.3/bin/VarDict -G "+refGenome+" -f 0.001 -N "+sample+" -b "+cleanBqsrBam+" -c 1 -S 2 -E 3 -g 4 "+targetBedFile+" | teststrandbias.R | var2vcf_valid.pl -N "+sample+" -E -f 0.001 > vars.vcf"
Even so, it doesn't detect neither teststrandbias.R, nor var2vcf_valid.pl, which in theory, are inside the /bin/VarDict folder. I'm working from a server and I don't know how to fix it.
Also, if you think I should change any of the arguments to optimise the search for variants in paraffin samples that are not of high quality, any advice would be welcome!
Thanks in advance to all of you!
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What does the following mean?
How is
bashArgumentsbeing used?I am using it by using sbatch, and calling the .py script it contains:
But even so, it does not detect the .R or the .pl I try to call when I use the basharguments. In the /apps/VARDICTJAVA/1.8.3/bin> folder, there is only: VarDict VarDict.bat.