How to solve, Error, the column headings: are not unique. in Build Transcript and Gene Expression Matrices
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9 months ago
mavs_007 • 0

I'm trying to generate Build Transcript and Gene Expression Matrices, my command is:

 /trinityrnaseq-v2.13.2/util/abundance_estimates_to_matrix.pl --est_method salmon Sy_Externa_1/quant.sf Sy_Externa_2/quant.sf Sy_Externa_3/quant.sf Sy_Externa_4/quant.sf Sy_Externa_5/quant.sf Sy_Externa_6/quant.sf Sy_Externa_7/quant.sf Sy_Interna_1/quant.sf Sy_Interna_2/quant.sf Sy_Externa_3/quant.sf Sy_Externa_4/quant.sf --gene_trans_map none --name_sample_by_basedir

* Outputting combined matrix.

But keep coming the same Error, the column headings: Sy_Externa_1 Sy_Externa_2 Sy_Externa_3 Sy_Externa_4 Sy_Externa_5 Sy_Externa_6 Sy_Externa_7 Sy_Interna_1 Sy_Interna_2 Sy_Externa_3 Sy_Externa_4 are not unique.  Should you consider using the --name_sample_by_basedir parameter? at /trinityrnaseq-v2.13.2/util/abundance_estimates_to_matrix.pl line 248.

Please anybody could help?

trinityrnaseq rna-seq • 503 views
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You have the same file more than once in the command line

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