Using tRNAscan-SE for mitochondria tRNA from microeukaryotes like protists and yeast?
I'm working on calling tRNA genes from the mitochondria of protists and yeasts using tRNAscan-SE 2.x
Here's the search modes available:
Search Mode Options:
-E : search for eukaryotic tRNAs (default)
-B : search for bacterial tRNAs
-A : search for archaeal tRNAs
-M <model> : search for mitochondrial tRNAs
options: mammal, vert
-O : search for other organellar tRNAs
-G : use general tRNA model (cytoslic tRNAs from all 3 domains included)
--mt <model> : use mito tRNA models for cytosolic/mito detemination
(if not specified, only cytosolic isotype-specific model scan will be performed)
For mitochondria should I use -M vert, -O, or -B?
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1 answer
You should use -O.
-M and -B are for other taxons.
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