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Query Regarding getting same value for start and end position, "DNA methylation"

Hi everyone,

Greetings!

While using methyl kit for Bisulphate sequenced data, I am getting similar start and end positions. Please help me to find a way to get different start and end positions.

Thank you in anticipation.

dnamethylation methylkit

Your question lacks precision, we do not know what you are talking about

Are you working on bisulphite kind of data or chipseq ????

That's a comment, not an answer, please use ADD COMMENT.

yes I am working on Bisulphate sequenced data.

Yes, that is what methylkit is for. See my answer.

1 answer

genomation is based on GenomicRanges which uses 1-based coordinates. Cheat Sheet For One-Based Vs Zero-Based Coordinate Systems

Since a single CpG has a length of 1 you would get the same start and end for a single nucleotide, like chr1-1-1 so on chromosome 1 starting and ending at the first base.

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