When I wrote t-test to check if the cancer samples are significantly different than the normal samples using R I was thinking whether I should add a sentence encouraging you to google your problems first but I did not. Now I do. All these standard questions have been asked before. rlog and vst are even covered in the DESeq2 manual and this has been asked many times before. You have asked many questions here over the last two years. Nothing basically wrong with that, but an essential skill to build a career in bioinformatics relies on finding solutions and answers without spoonfeeding from others. Use search engines, read manuals and papers, do research yourself. You cannot expect strangers on the internet to guide you through your analysis. There are a few users here who assume biostars was a personal helpdesk . Please build the essential skills to find answers yourself, especially if questions have been asked before, don't become one of these lazy users who shotgun questions. Pretty much all relevant questions related to the strategy in the other thread have been asked before, please go find the answers, that is also a learning experience. There is in the longterm no value in getting spoon-fed.