Hello, Does anyone have any experience extracting unmapped reads from BAM files and converting them back to fastq format using Rsamtools? If you could provide …
I have been given the following command to extract unmapped reads from a bam file samtools view -b -f 141 ${SAMPLE_ID}.ecoli.bwamem.bam > ${SAMPLE_ID}.unmapped_ecoli_2.bam 1. I …