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Gene Set Variation Analysis (GSVA)

Hello,

I am trying to run GSVA and have two questions.

1) Are the normalized enrichment scores (NES) independently calculated for each sample? I subsetted my dataset and the NES values differed a bit for individual samples. I.e., can I run GSVA on a single sample?

2) Is there any way to get regular enrichment scores (ES) in the output, rather than normalized ES? In the GSVA R package, there is a way to do this for ssGSEA, but I am wondering for GSVA specifically.

Thank you so much.

microarray gsva rna-seq

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