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Error while running GATK4 Mutect2 in Galaxy

I have one clown and one control sample of python molurus bivittatus. After alignment, I am trying to use GATK4 Mutect2 to joint variant calling: cl vs ctrl

But after executing I got this error:

A USER ERROR has occurred: Bad input: The given bam input has no sample names.

How can I solve it?

mutect2 gatk4

1 answer

bam is missing read groups: https://gatk.broadinstitute.org/hc/en-us/articles/360035890671-Read-groups

so how can i add read groups?

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