Inferring relative gene abundance from short reads
Hi all!
I'm doing some metagenomics analysis (Short read) and would like to get the relative abundance of certain antibiotic resistance genes to total 16S. I was thinking on mapping the reads to a specific database and then mapping the reads against the 16S gene. Finally I was thinking on dividing reads_mapping_gene/reads_mapping_16S to get a relative abundance. Does that sound like a sensible approach? I feel there's something I'm not considering.
Thanks!!!
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SushiRoll •
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