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How to retain proteins with both start and stop codons, and remove those with internal stop codons from FASTA file ?

Hello, Curious to know if anyone knows how to retain proteins with both stop codons and remove those with internal stop codons from FASTA file?

I noticed a number of internal stop codons in the minprot generated output file and wanted to filter them out. Kindly suggest!

Regards, B

gene annotation

1 answer

You run a gene prediction program such as Prodigal and there won't be any predicted proteins with internal stop codons.

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