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How to know the sample total read in SRA

Hello there,

I have a project that I should select sample that has from 25 to 50 million read how I know that? And to someone explain to me what spot and bases mean? When say the run SRR34558 has 28.8 M and bases 3.6 Gbp what I should understand from those numbers?

sra

Have you tried Googling these terms? Where exactly did you run into difficulties understanding them

Not exactly about the terms, it’s about that I should find sample with average 25 to 50M reads so I was wondering if the spot number is actually the total number of reads in the sample or where exactly I should search for it to know?

1 answer

Concept of a spot is probably deprecated at this time but it used to mean this in days past (What Is A "Spot" In Sra Format ).

For current data those numbers should mean what they say. There are NN million reads adding up to NN gigabases.

So what you mean that the total number of spots is total number of reads right?

Yes, that's what he's saying. It's NN million reads or pairs of reads depending on SE/PE protocol. I just verified it using a sample I uploaded for my lab.

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