so if I just consider one of the SRR and use fastq-dump --splite -files , its okay ???
RNA-SEQ
Hi everyone, I am having issues with my RNA-seq data. Specifically, I have data for a single sample that includes two different SRA numbers. Although my data is paired-end, I am uncertain whether each SRR number corresponds to the forward or reverse reads, or if they represent technical replicates. Could someone provide clarification on this matter? SRR10239199 and SRR10239200 for GSM4110156!
• 1,744 views
•
link
1 answer
Those are replicates, and if you download the SRR files, you should automatically get R1 and R2 for each SRR.
• 0 views
•
link
• 0 views
•
link
If you are trying to re-create an analysis and if it used both SRA# then you will need to get both. If you are simply learning/testing then one of SRR may be enough.
• 0 views
•
link
Log in to answer this question.