Do we need replicates for PSI calculation in SUPPA2?
Hi,
I don't have replicates for my RNA-seq experiment. However, I have 6 samples and six controls from different plants. I am trying to perform Alternative splicing analysis and have generated All splicing events using SUPPA2 event generator. However, I am not able to calculate PSI from individual TPM values. What should I do?
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sreelekha •
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Have your tried
psiPerEvent? https://github.com/comprna/SUPPA#psi-per-local-eventThank you for the link. Yes! I got the psi values but I guess differential expression and clustering cannot happen without PSI values.