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resequensing of the whole sample or sequencing to X k reads and merging files

Dear community,

I have sequenced my RNAseq library for example just to 30M of reads. When I checked transcripts of interests I realized that I would like to have a higher depth. The sequencing center offers me to sequence another 30M of reads and then merge files. Is it an appropriate solution? Would it be better just to sequence again to 60M of reads? If I sequence again just to 30M of reads are these just technical replicates or new sequencing (to this 30 M + merge) might bring additional information?

Thanks in advance for the response.

rna-seq sequencing-depth
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updated by Ram Ram • written by esimonova.me • esimonova.me

1 answer

There is literally no difference between the two approaches other than the 2nd one being more expensive. Reseq and merge is fine and standard.

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written by ATpoint ATpoint

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