Could someone provide a detailed guide on downstream preprocessing steps based on my FASTQC report? I conducted FASTQC analysis on a paired sample, and here …

How can I remove warnings for per base sequence content, per sequence GC content and sequence duplication levels using trimmomatic or fastp. Thanks ![enter image …

Hello, I conducted some metagenomic sequencing as follows: - Metagenomic sequencing from human stool samples - PCR-free library prep using NEB kit (450bp insert) - …

Hi, I use the following code to get the heatmap (figureA): **My code:** ![enter image description here][1] **Figure A** ![enter image description here][2] However, I …

Hi I am doing a Chip-seq analysis. How do you cut for base sequence content in the final part where some bases tend to go …

Hi All, After I use fastp to preprocess the reads, the fastqc report became worse. It seems that the reads have been preprocessed since the …

I'm running FastQC on some paired-end fastq files. I have a warning on per-base sequence content, as the first 5 to 6 bases show significant …

Here is the Per base sequence content part of my sample's fastqc report. It was marked 'fail'. ![enter image description here][1] Will it influences my …

In my RNA sequencing fastqc report, I consistently notice an abnormally high A (green) peak in session "Per base sequence content". See images below. I …

<p>HI, I have a question here, my reads have over all good per base and per sequence quality but there are three potential problems at …