If you are struggling to append gene-related features to a GTF file converted from a BED file this tool could be an option: bed2gtf. Just need an isoforms file to map each line and append gene features.
I am trying to run the python package how_are_we_stranded_here on my university's supercomputer to use the check_strandedness function. https://github.com/signalbash/how_are_we_stranded_here#readme I was able to successfully install …
Hello, I have a FASTA file that has reads like this: >\>SRR5655563.745 745 length=126 (type=T,start=2,end=12,length=11,identity=81.8182%) CttttatttgttGTGTGTGAGGTTTGATTTGATGGGAAAATATCTTGAATCTGCGGCGAGGTTGGAAGAGCTATCGCGGATTGTGTCATCTGCTGCGAAGCCCAATAGGTCAAAGGGAACGCTACC As you can see, there is information about the …
Hello, I have been trying to create a kallisto index using the following command: kallisto index -i Glycine-Max.idx Glycine_max.Glycine_max_v2.1.cdna.all.fa.gz It does run but soon encounters …
Hello kind folks, I have mapped some long-read mouse sequencing to the mouse cDNA sequences. I now have a list of Ensembl transcripts (e.g. ENSMUST00000232790.2) …
Download from the UCSC Table Browser or use the respective converters in the binary utilities:
GTF/GFF3 -> GenePred -> BED.