Hello. Can anyone explain to me what they mean by "a corresponding gtf" and where do I get it from? I'm super new to this stuff and have trouble understanding which files are which.
It also requires a transcriptome annotation (.fasta file - e.g. ensembl’s .cdna.fasta, or a prebuilt kallisto index), and a corresponding gtf.
And for salmon, in the following command:
salmon quant -i <transcriptome index> --libType A \
-o <out dir and prefix> -1 test_1.fq.gz -2 test_2.fq.gz
-p <assigned threads>
Where do I get a transcriptome index for a SRPxxxxx?
Edit: I'm working with RNA-Seq data
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pubsurfted •
1 answer
download fasta and gtf here (if working with human): https://uswest.ensembl.org/Homo_sapiens/Info/Index
You can't use kallisto or salmon unless you build an index for them and typically an index requires a FASTA (e.g. one you'd download from the link above). The "getting started" tutorials for either tool shows you how to build an index.
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