Hello Biostars users,
I am trying to map two different Seurat objects with three samples each. Both the Seurat object came from the same sample but with different downstream analyses. I am interested in mapping them cluster-wise to get what cells were clusters together. I tried every option from integrating the datasets to finding anchor but nothing seems identifying the cells mapped with each other. Is there a way to perform such an analysis?
1 answer
I am not sure exactly what you are trying to do, but I doubt you need to use any anchors or integration since you are looking at the same exact cells. Both objects have a metadata slot (obj@meta.data). You can take the relevant column from both objects and then compare for each cell.
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