Reanalyzing data after Seurat subset
I'm following the Seurat WNN workflow to analyze my multimodal data. I am subsetting the object to exclude one of the clusters. After removing these cells, are there any steps in the workflow that I need to repeat (normalization, finding variable features, scaling, etc)?
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It depends on what you are trying to do, but you should be fine by re-clusterin you dataset (
RunPCA->FindNeighbors->FindClusters).