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To create a pre-trained classifier provided by QIIME2 using RESCRIPt

Hello all,

I would like to use Rescript to create the following pre-trained classifier provided by QIIME2.

(pre-trained classifier)Silva 138 99% OTUs full-length sequences (MD5: b8609f23e9b17bd4a1321a8971303310)

I have created a classifier according to the Tutorial at the following site, but I am not sure how far I need to go to create exactly the same as the pre-trained classifier provided by Qiime2.

If anyone has any advice, I would appreciate it if you could open it.

Thank you in advance.

rescript rrna 16s qiime2 silva

To fix the problem you were describing in this post you should run the following chunk of code with the option --p-no-rank-propagation

qiime rescript parse-silva-taxonomy \
    --i-taxonomy-tree taxtree-silva-138.1-nr99.qza \
    --i-taxonomy-map taxmap-silva-138.1-ssu-nr99.qza \
    --i-taxonomy-ranks taxranks-silva-138.1-ssu-nr99.qza \
    --p-no-rank-propagation \
    --p-include-species-labels \
    --o-taxonomy silva-138.1-ssu-nr99-tax.qza

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