Thank you very much.
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Dear all,
I used RNA STAR from Galaxy and got 5 files.
The reads per gene file contains 4 columns, but they dont have any annotations.
What is the difference among the last 3 columns?
Thank you in advance, Anton
https://physiology.med.cornell.edu/faculty/skrabanek/lab/angsd/lecture_notes/STARmanual.pdf
STAR outputs read counts per gene into ReadsPerGene.out.tab file with 4 columns which correspond to different strandedness options: column 1: gene ID column 2: counts for unstranded RNA-seq column 3: counts for the 1st read strand aligned with RNA (htseq-count option -s yes) column 4: counts for the 2nd read strand aligned with RNA (htseq-count option -s reverse)
Thank you very much.
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