Sure, but why not use the information from the one member of the pair to help map the other? You can map them together, then filter the bam to pull out the all the read1 reads.
Hi there, I have successfully created an annotated genome index to map my paired-end rna-seq reads and have run: STAR --runMode alignReads --genomeDir /home/rnaseq/ref/ --outSAMtype …
<p>I have illumina paired-end whole-genome sequencing reads which I have map to around -400 reference plastid genomes. After getting mapped reads, I have to assemble …
Yes. Just map each one individually (I would not recommend it but it's possible)