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Can't create index file of Bam file

Hello,

I am trying to create a index file of file.bam using Samtools. I have executed this command :

samtools index file.bam  file.bam.bai

But it shows some error while creating this index file. I have attached my screenshot bellow: it shows some positions cannot be indexed

Anyone can help me about how can i get rid of this error?

bam samtools

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