SARS-Cov-2 genomics analysis
Hello everyone,
I want to analyse the SARS-Cov-2 genomics. But for the amplicon-based method, is it necessary to know the primer sequence for each sample, no matter whether it is done by Illumina or nanopore? Thanks
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Arup Ghosh
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kimmitzka •
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kimmitzka •
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Most of the library preparation kits the ARTIC primer sets and to remove any artifacts introduced by the non-genomic sequences trimming of respective primer sequences is essential for any kind of amplicon sequencing.
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From your question, it is not clear (a) what you have done [and possibly hit an error] and (b) what type of analysis you want to achieve