The first amplicon librariesis is needed to analyse the 16S data?
Hi,
For analyzing 16S data with Mothur or Qiime is it necessary that I have the the first amplicon libraries sequences or not? The data is produced by MiSeq illumina multiplexing technology and the barcode of each sample is provided by the lab.
Best
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Hi,
if you are looking for OTUs from sequencing data in order to compare microbial communities in your samples, you will only need FastQ files from sequencing.
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