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Demultiplexing paired-end illumina data with separated R1 file, R2 file and index file

I need help demultiplexing paired-end fastq files. I was given an index file, an R1 fastq, an R2 fastq, and the barcode spreadsheet. Is there any way to do this on R?

demultiplex

What kind of lazy sequencing facility goes through the work of making your fastqs without demultiplexing for you?

1 answer

Not in R but you could do this using a program called demuxbyname.sh in BBMap suite. See an example --> demuxbyname.sh output help

Other option would be to use a program like deML: https://github.com/grenaud/deML

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