Demultiplexing paired-end illumina data with separated R1 file, R2 file and index file
I need help demultiplexing paired-end fastq files. I was given an index file, an R1 fastq, an R2 fastq, and the barcode spreadsheet. Is there any way to do this on R?
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Not in R but you could do this using a program called demuxbyname.sh in BBMap suite. See an example --> demuxbyname.sh output help
Other option would be to use a program like deML: https://github.com/grenaud/deML
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What kind of lazy sequencing facility goes through the work of making your fastqs without demultiplexing for you?