I referred paper named 'CRISPR-Cas9-Based Discovery of the Verrucosidin Biosynthesis Gene Cluster in Penicillium polonicum' to find out certain gene cluster from my genomic analysis data.
They utilized antiSMASH to get gene cluster and I conducted same process, but I couldn't find same gene cluster, and I found out another process.
'To identify BGCs in the genomes of P. polonicum strain IBT4502 (GCA_002072265.1) and P. polonicum strain hy4 (GCA_003344595.1), antiSMASH (Weber et al., 2015) was used and only clusters containing a putative PKS similar to both CtvA protein (Q0C9L7.1) and AurA (A0A0M4L8I7.1) were further considered (query coverage ≥50% and e-value > e–5).'
They run antiSMASH and additionally found gene clusters which contain certain protein sequence (CtvA and AurA).
Those antismash can make it, or another program is required?
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They probably used blast or similars to search for CtvA and AurA homologs only in PKS gene clusters.