How to do differential expressions in R from scRNAseq count files after scaling and normalization? · Biostar

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How to do differential expressions in R from scRNAseq count files after scaling and normalization?

I have control and treatment data to be analyzed. I did the sailing, normalization and integration following Seraut. I got 11 clusters from integrated data and now I wanna make heatmap of differentially expressed genes, however not all genes but few selected genes. Can anyone please help me with the codes and explanations?

Thanks

r scrnaseq de

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updated 4.1 years ago by jared.andrews07

• written 4.1 years ago by Pankoj •

1 answer

Read Seurat's DoHeatmap documentation. Or dittoSeq's dittoHeatmap documentation, either will work for whatever genes you want to plot and are very easy to use.

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written 4.1 years ago by jared.andrews07

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