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Identifying DEG from time course data without condition

Hi,

I have a a time course data for 7 time points with 3 replicates each and I would like to point out the clusters of genes that are differentially expressed in at least one group Vs others.

Similar problems with control and treatment problems can be solved with DESeq2 but I don't have 2 condition model.

Kindly, let me share your knowledge.

Thank you.

deseq edger rna-seq
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updated by Benn Benn • written by ChIP ChIP

Section 3.4 in DESeq2 vignette tells you how to analyze time series data.

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written by GenoMax GenoMax

1 answer

If you use edgeR, you can follow the ANOVA-like test in the manual.

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written by Benn Benn

Hi,

I am also trying to compare multiple samples by EdgeR with ANOVA-like approach.

I would like to get all the FC and P-value, FDR for all comparison.

After looking on forums and reading the manuals, I am still not able to obtain the information which comparison is significant, is there a way to do this? By following the manual I am only getting FC values for the different comparisons and a single P-value, FDR. Am I missing something?

Thanks you.

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written by hlsz.laszlo • hlsz.laszlo

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