Bimodal read lenghts in pacbio transcriptome sequencing
Hi guys,
I am analyzing the data sequencing of a RNA-Seq using Pacbio. It was done with two cells (one with < 5kb, and the other 5-10kb) of sequencing. When analyzing the transcript lengths, it produces a bimodal distribution with hardly an overlap between the two modes and one peak at 1 kb and the other at 5.5 kb. Has anybody had the same pattern when analyzing pacbio transcriptome sequencing data?
Best regards,
Carlos
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