metagenome binning using MaxBin2 for each dataset
Hi! In the documentation of MaxBin2 it is written that it is used for binning of the co-assembly of many metagenomic datasets. But I did assembly for individual sample using MegaHit and the final contig from each sample is used as input for the MaxBin. Is it wrong to use the contig from single dataset assembly as input for the maxbin? or it is only correct when I am giving the co-assembly results to maxbin for binning?
Thanks in Advance
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While the authors claim that you get better results with co-assembly, you can still use MaxBin2 for single sample assemblies.
Other binning tools you should look at are MetaBAT2 and semibin. In my experience these tools outperform maxbin2.
Alternatively, you could try the
Bin_refinementmodule from metaWRAP: