Hello to everyone! I have the results of some RNA Seq analysis and I am trying to apply over representation analysis using clusterprofiler. I get the DEGs and then I divide them to upregulated and downregulated. Next I use the clusterprofiler to apply the overepresentation test but the result of a set of one upregulated gene set and one downregulated gene set gives back zero enriched terms. I am trying to get a goplot (goplot()) and enrichment maps (emapplot()) and in the instance of the downregulated gene set although I cant get a goplot I can get the enrichment map. My question is if that is normal sometimes and if not where i am mistaken. Also how is it possible to create the enrichment map aout of no enriched terms? Thanks in advance!
0 answers
No answers yet.
Log in to answer this question.
How many upregulated and downregulated genes do you have?
Hello thanks for replying. I have 172 downregulated and 216 up regulated. Note that these are from different expreimnts and not from the same.