This is a repository for a project that uses tax4fun2, not the repository that documents T4F and provides it for download/installation. All repositories for installing T4F2 and T4F seem to have been removed from the internet in the last few months.
Hi everyone!
I have tried to find the R package Tax4Fun2 from the paper (https://pubmed.ncbi.nlm.nih.gov/33902725/) . This R package lets analizes the microbiome in an easy way to predict functional profiles from metagenomic 16S rRNA data.
However, I have not found the package in R CRAN packages, neither in the github refered in the original paper (https://github.com/bwemheu/Tax4Fun2) or the Tax4Fun page (http://tax4fun.gobics.de/).
I am looking for Tax4Fun2 because gives higher accuracies than PICRUSt and Tax4Fun.
Any help will be grateful!
Best,
E
2 answers
I searched GitHub for Tax4Fun2 and found this:
Hello , I also have the same problem. Did you find repositories for installing T4F2 and T4F please ? if not what did you use as an alternative and are you satisfied with the results of the alternative tool ? please answer !!
https://github.com/ZihaoShu/Tax4Fun2 has tar file Tax4Fun2_1.1.5.tar.gz, download and install from source.
install.packages("Tax4Fun2_1.1.5.tar.gz", repos = NULL, type = "source")
Hello! I was confused as well.
When installing Tax4Fun2, Follow this: https://github.com/songweizhi/Tax4Fun2_short_tutorial?search=1
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They tested Tax4Fun2 (2020) against PICRUSTt version 1, which is very old (2013). In this paper on the other hand, Tax4Fun2 actually performs worse than PICRUSt2
Hello , I also have the same problem. Did you find repositories for installing T4F2 and T4F please ? if not what did you use as an alternative and are you satisfied with the results of the alternative tool ? please answer !!
please I am trying to install and use Tax4fun2. but do you have the SILVA_132_SSURef_NR90.fasta file? I attempted to locate it in the Silva Database, but I couldn't find it.
https://www.arb-silva.de/no_cache/download/archive/release_132/ARB_files/
Tax4Fun2 requires the SILVA_132_SSURef_NR90.fasta file specifically... Thank you so much
You just need to look through other directories. This file is the closest to what you want:
https://www.arb-silva.de/fileadmin/silva_databases/release_132/Exports/SILVA_132_SSURef_Nr99_tax_silva.fasta.gz
After unpacking, you can either rename the file to
SILVA_132_SSURef_NR90.fastaor change the code to use the file you have.Thank you so much. I downloaded it and renamed it, now I need to download and install prodigal_v2.6.3
Thank so much
I have downloaded it from here: https://mirrors.opensource.is/ubuntu/pool/universe/p/prodigal/
prodigal_2.6.3.orig.tar.gz
but there is no file named: prodigal.macos
what should i do? I have Tax4Fun2_ReferenceData_v2/TOOLS/prodigal_v2.6.3/prodigal.macos (renamed it prodigal), Tax4Fun2_ReferenceData_v2/TOOLS/diamond_v0.9.24/diamond.macos (renamed it: diamond)
Mai Abdelmonem : Stop posting errors and comments as new answers.
Submit answeris only for new answers to the original question.Okay, I see. Sorry for that