Minimal insert size for Illumina MiniSeq
Hi everyone
What is the minimal insert size that can be reliably sequenced in Miniseq?
Is it okay to design a target DNA panel that has a region of interest (ROI) only 3bp long? Then, the insert size is going to be only between 50-70bp.
The intended application is target variant detection.
Thanks a lot in advance
Milos
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
An alternative to haplotype phasing - assembly?
written by Jess •
-
Libraries with different size in the same MiniSeq cartridge
written by MH85 •
-
fgbio read structure
written by MH85 •
-
About 10x chromium Gene assembly
written by r00628112 •
-
GATK HC not calling variants at the edges after clipping probes
written by Floydian_slip
-
Amplicon sequencing: how to handle adapters and primers?
written by lamteva.vera
-
Insert size calculation for de-novo assembly
written by ram.mainali •
-
how sequenced? dna between the pair-end reads
written by adamselwithadulcimer •
-
Beta-values, M-values and thresholds on effect size
written by gbayon
-
Depth of RNAseq with sensitive detection
written by sckinta
