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fgbio read structure

Hi everyone,

could anyone tell me the correct syntax of fgbio’s Read Structures for 150bp-PE reads generated from QIAseq® Targeted DNA Panel, please? I have demultiplexed fastq data from MiniSeq, UMIs are 12bp long and there is 11bp common sequence that comes after UMIs.

Thanks in advance

Milos

fgbio umi extractumisfrombam

1 answer

Use this tool for vardating read structure: https://fulcrumgenomics.github.io/fgbio/validate-read-structure.html

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