qPCR Assay plot
Dear all,
As part of my qPCR analysis, I performed two qPCR assays for the same samples using two different protocols. I have two data frames with rows of samples and columns of RNAs. The rows and columns have the same names in both data frames.
I don't know how I can compare each CT value between the two data frames shown in a barplot or other plot.
I would appreciate any solution or recommendation.
Best Regards
Maria
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Clustering method for CT values of two group
written by Maria17 •
-
Normalization In qPCR assay
written by Maria17 •
-
qPCR assay Triplicate Ct SD
written by Maria17 •
-
qPCR result for Technical replicates
written by Maria17 •
-
add patient information to the metadata or Make New Orig.ident in seurat object
written by Maria17 •
-
determine the robustness of single-cell clusters
written by Maria17 •
-
Cluster Stability Assesment
written by Maria17 •
-
Estimation of Copy Number of alterations(CNA) in Sc-RNA Seq data
written by Maria17 •
-
problem in visualization of Gene names instead of Gene ids in annotated Seurat object
written by Maria17 •
-
Imputation of missing values for RT-qPCR data
written by mohammedtoufiq91
I don't think you have a clear idea of the experimental goals of your qPCR experiment so I'd recommend taking a step back. Are you trying to compare the results from the two different protocols? Are the two conditions done in a similar enough way to even compare? Are you planning on doing dCt or ddCt?
Dear Trivas,
it is the dCt, i want to compare pPCR output of two different protocol ,the samples are the same. basically comparing the number of dCt from two protocol & plotting in a bar graph or correlation graph.
Regards M