Spades assembly error
Hello, I am using spades for WGS data, plz let me know how can i resolve these warning, I am using this script
spades.py -t 30 -1 ER2_1.fastq.gz -2 ER2_2.fastq.gz -o "/RAD_F2_186"
======= SPAdes pipeline finished WITH WARNINGS!
=== Error correction and assembling warnings:
* 0:05:35.557 188M / 897M WARN General (kmer_coverage_model.cpp : 327) Valley value was estimated improperly, reset to 1
* 0:05:35.558 188M / 897M WARN General (kmer_coverage_model.cpp : 366) Failed to determine erroneous kmer threshold. Threshold set to: 1
* 0:05:08.084 150M / 1174M WARN General (kmer_coverage_model.cpp : 327) Valley value was estimated improperly, reset to 8
* 0:05:08.086 150M / 1174M WARN General (kmer_coverage_model.cpp : 366) Failed to determine erroneous kmer threshold. Threshold set to: 8
* 0:03:14.959 194M / 1068M WARN General (kmer_coverage_model.cpp : 218) Too many erroneous kmers, the estimates might be unreliable
* 0:03:26.284 194M / 1068M WARN General (kmer_coverage_model.cpp : 327) Valley value was estimated improperly, reset to 7
* 0:03:26.285 194M / 1068M WARN General (kmer_coverage_model.cpp : 366) Failed to determine erroneous kmer threshold. Threshold set to: 7
* 0:05:15.442 266M / 1068M WARN General (simplification.cpp : 500) The determined erroneous connection coverage threshold may be determined improperly
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google:
Failed to determine erroneous kmer thresholdThey're just warnings. You don't necessarily have to do anything about them if your output assembly looks reasonable.