How to do transcriptome annotation through transcriptome annotation gtf file obtained after cuffllinks?
Hello Researchers
Teacher gave work to do transcritpome annotation with only transcriptome annotation GTF file which was obtained after cufflinks of 2 expression data control and test, the GTF file only contains exon.
How to find all annotation from these after cufflinks GTF file
promoter/cds?
Work I tried already : from reference genome GTF file I separated all annotated regions and extracted sequences than did blastn with transcriptome sequences and took top most hit, but teacher told focus on only transcriptome sequences for annotation, I am confused, please help me.
Thank you
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