This is a test version of Biostars. For the public version, visit https://www.biostars.org.
How to do transcriptome annotation through transcriptome annotation gtf file obtained after cuffllinks?

Hello Researchers

Teacher gave work to do transcritpome annotation with only transcriptome annotation GTF file which was obtained after cufflinks of 2 expression data control and test, the GTF file only contains exon.

How to find all annotation from these after cufflinks GTF file

promoter/cds?

Work I tried already : from reference genome GTF file I separated all annotated regions and extracted sequences than did blastn with transcriptome sequences and took top most hit, but teacher told focus on only transcriptome sequences for annotation, I am confused, please help me.

Thank you

gtf transcriptome annotation

The idea of assignments is to figure it out yourself. At least present some outline of ideas.

0 answers

No answers yet.

Log in to answer this question.