How does BUSCO convert MetaEuk identifiers?
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Entering edit mode
2.2 years ago
O.rka ▴ 720

I'm trying to understand the logic of converting the complex MetaEuk identifiers to the more simple identifiers adopted by BUSCO so I can reproduce a manual step for gene calls prior to BUSCO. However, what I thought was the case isn't actually correct. I thought you just get the high and low values.

>Transcript_22646|m.42971|NODE_14273_length_2360_cov_2.257701|+|428|5.129e-119|1|98|742|98[98]:742[742]:645[645] 
VPVFIMPLFNKYEPLDDGTLKTRIYELAASLKYPLTKLFVMDGSKR...
>Transcript_22646|m.42972|NODE_14273_length_2360_cov_2.257701|-|290|1.787e-77|2|98|742|742[742]:356[356]:387[387]|283[283]:98[98]:186[186]
HQGRMTVMIKRIILVRVHGAQVFQMNLAQTTLQLFGPTQINRKAIG...

Both of these (with my incorrect logic) would return the following: NODE_14273_length_2360_cov_2.257701_98:742

calls metagenomics gene eukaryotic metatranscriptomics binning • 470 views
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1
Entering edit mode
2.2 years ago
O.rka ▴ 720

This was answered elsewhere by the author: https://gitlab.com/ezlab/busco/-/issues/539#note_878112269

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