I have RAW Illumina Paired-end sequence in FASTQ format. Can someone please guide me on how to create a single FASTA file for analysis? I want to check the presence of bacterial resistance. Need help
I have RAW Illumina Paired-end sequence in FASTQ format. Can someone please guide me on how to create a single FASTA file for analysis? I want to check the presence of bacterial resistance. Need help in this.
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swbarnes2
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mibtisamnasar •
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mibtisamnasar •
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You can use velvet or spades for reads assembly, then use blast on the obtained FASTA to know to which bacteria your samples resemble.
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It's not a pushbutton thing you are asking. Have you googled a variant calling tutorial?