Hi friends

I am trying to design a project based on DNA sequencing. I have problem with methodology that I can use.

I have a phenotype associated with a cancer and I want to screen this in PDX mouse model. After injecting tumor from patients with and without the phenotype, how can I compare the molecular features of my groups?

Is it possible to do DNA sequencing to understand the differences in genomic features of the groups?

Also, in order to have enough sample size, how many mouse should I use (how many patients should I use to inject from them to mice?)

What other suggestion you have that I can use?

Thank you for help