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interchromosomal interactions and 4C

Dear all,

would you please let me know, based on your experience, how reliable are the inter-chromosomal interactions called by 4C techniques ? Thank you.

~ Bogdan

4c
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written by Bogdan Bogdan

Bogdan - I would be happy to, but could you please describe (approximately) your goals for the experiment? It will help me to describe the reliability.

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written by LauferVA LauferVA

Thank you Vincent. I would like to evaluate whether 4C can capture the inter-chromosomal contacts that have been characterized in MCF7 cells after estradiol treatment. For example, in the publication :

https://www.pnas.org/content/105/49/19199.long

the authors describe the interactions between TFF1 and GREB1 gene loci that are located on different chromosomes (figure 4). Thank you.

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written by Bogdan Bogdan

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