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cellranger for single nucleus RNA sequencing

Hello :)

When I use cellranger for snRNA-seq data, it is confusing whether output is correct or not. enter image description here

I used '--include-introns' parameter because we should detect pre-mRNA. In web summary, however, we can see the error message for 'Fraction Reads in Cells' section. In 10X genomics Q&A, we should use '--include-introns' parameter because 10X genomics do not serve reference for snRNA-seq. So, it this web summary normal?

genomics cellranger 10x snrna-seq
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written by dahun73 • dahun73

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