Hi friends I am trying to do BLASTn in galaxy. I have one query gene(my reference fasta file) and two bins containing contigs (my subjects). one of my blast result is empty with 0 line in it. why is this? Should I do this one by one? becuase I did blastn for tow bins same time. this is the screenshot:
Rob,
no significant hits were found in the bin 002. That's it.
Why would you expect a different result?
thanks Before doing BLASTn I did bining and I got two bins. the result is showing this in one of the 4 output in the right menu: https://ibb.co/PCDQnjX
can the blasting be because this has 0 line? if yes, what is the reason for getting this when bining?
0 lines = 0 hits = no significant alignments were found
This has nothing to do with the binning. The fact that both bins are classified as Bacteroides does not necessarily mean that they have to share the same sequence content
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