How to specify the depth of sequencing in ion torrent?
I have never used ion torrent machine before, but in Illumina there is a calculator to specify the coverage before sequencing. Is there for ion torrent a similar thing, or in other words, can it be controlled in ion torrent sequencing ?
1 answer
The coverage of a sequencing run is the size of the genome divided by the number of total bases sequenced.
When an instrument produces identical read lengths then the formula is
coverage = number_of_reads * lenght_of_each_read / genome_size
The genome size is the expected length of the DNA that gets fragmented.
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