Ancient DNA Analysis Fastq to vcf

When combining multiple sample into vcf file, is it necessary that all samples must be aligned to the same reference build version ? such as Grch38, 37 etc.,

vcf genotyping

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updated 3.4 years ago by Ram

• written 4.5 years ago by Aruna •

1 answer

Dear Aruna,

Yes! You will have to do a liftover from one build to another before merging into a single VCF

All the best

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written 4.5 years ago by tacrolimus

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