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Hypothesis testing of multiple genes for multiple samples

If I have an x number of genes for n number of samples with values representing gene expression values for cancer. And I have the same data for a healthy individual and I want to check if the change is significant! How do I put it in hypothesis terms? Also, what’s a fold change? And can it be of use in this example?

fold_change hypothesis_testing

This sounds like a homework question.

well I am only asking how would you approach such problem, so it's more of a guiding advice.

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