How to perform multiple sequence alignment with large nucleotide sequence
Hi, I have a fasta file of 30 nucleotide sequences of the viral genome, and am looking to perform multiple sequence alignment on these sequences. Then I want to construct the phylogenetic tree between these sequences. I tried the MEGA X GUI version but it is unable to perform that large .fasta file. Please suggest any aligner that I can use and perform a phylogenetic tree at MEGA.
Thank you!
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